Journal:

Article Title: Divalent ion block of inward rectifier current in human capillary endothelial cells and effects on resting membrane potential

doi: 10.1111/j.1469-7793.1998.119bf.x

Figure Lengend Snippet: A, current traces from a HCEC recorded in response to voltage step pulses from −120 to +10 mV in 20 mV intervals at a holding potential of −60 mV. B, current traces from the same voltage pulse protocol after application of 50 μm Ba2+. C, current-voltage plot of peak (•) and steady-state (▪) current in standard bath solution, and peak (○) and steady-state (□) current after application of Ba2+ for the same cell. D, current traces from a HCEC response to voltage steps of −120 mV to +10 mV from a holding potential of −60 mV with 5.4 mm K+ in the standard bath solution. E, current traces from the same voltage protocol in the absence of external K+. F, peak current-voltage plot from the currents in D and E before (•) and after (○) removing all external K+. Elimination of external K+ completely blocks the inward current.

Article Snippet: Cell culture Human capillary endothelial cells (HCEC) were obtained from Cell Systems Corporation (WA, USA), and were cultured in growth medium for human capillary endothelial cells (4M1-500, Cell Systems Corporation).

Techniques:

Journal:

Article Title: Divalent ion block of inward rectifier current in human capillary endothelial cells and effects on resting membrane potential

doi: 10.1111/j.1469-7793.1998.119bf.x

Figure Lengend Snippet: A, inward rectifier current traces of a HCEC bathed in standard bath solution. The cell was held at −60 mV and step potentials were applied from −120 to +20 mV, in 20 mV steps. B, the same cell after superfusion with standard bath solution containing 9 mm Ca2+. C, addition of 50 μm Ba2+ blocked the remaining current. D, current traces taken after washout of high external Ca2+ and Ba2+ with standard bath solution. E, current-voltage plots for control, 9 mm Ca2+, 50 μm Ba2+ and after washout.

Article Snippet: Cell culture Human capillary endothelial cells (HCEC) were obtained from Cell Systems Corporation (WA, USA), and were cultured in growth medium for human capillary endothelial cells (4M1-500, Cell Systems Corporation).

Techniques: Control

Journal:

Article Title: Divalent ion block of inward rectifier current in human capillary endothelial cells and effects on resting membrane potential

doi: 10.1111/j.1469-7793.1998.119bf.x

Figure Lengend Snippet: A, effect of [Ca2+]o from 0.5 to 30 mm on the mean percentage block of peak inward rectifier current at −120 mV (n = 5). Inhibition of the current at different concentrations of external Ca2+ is well fitted by a logistic equation with an EC50 value of 5.4 ± 0.6 mm. B, representative current traces of a HCEC at −120 mV with [Ca2+]o from 0 to 9 mm. C, shifts in zero current level of a HCEC exposed to divalent cations. The cell was held at −60 mV and voltage ramps were applied from −120 to +20 mV. The zero current level occurred at −62 mV with 0.5 mm Ca2+, −47 mV with 7 mm Ca2+, and −18 mV with 50 μm Ba2+ in the bath solution.

Article Snippet: Cell culture Human capillary endothelial cells (HCEC) were obtained from Cell Systems Corporation (WA, USA), and were cultured in growth medium for human capillary endothelial cells (4M1-500, Cell Systems Corporation).

Techniques: Blocking Assay, Inhibition

Journal:

Article Title: Divalent ion block of inward rectifier current in human capillary endothelial cells and effects on resting membrane potential

doi: 10.1111/j.1469-7793.1998.119bf.x

Figure Lengend Snippet: A, current traces from a HCEC in Mn2+-free standard bath solution. The cell was held at −60 mV and pulsed from −120 mV to +20 mV in 20 mV steps. B, current traces after switching to a bath solution containing 5 mm Mn2+. C, the current-voltage plot of the same cell in standard bath solution and in bath solution containing 5 mm Mn2+. D, current traces (same voltage protocol as A) of another HCEC in standard bath solution without Mg2+. E, current traces after application of 5 mm Mg2+ in the standard bath solution to the same HCEC. F, plot of the current-voltage relationship of the HCEC before and after application of Mg2+.

Article Snippet: Cell culture Human capillary endothelial cells (HCEC) were obtained from Cell Systems Corporation (WA, USA), and were cultured in growth medium for human capillary endothelial cells (4M1-500, Cell Systems Corporation).

Techniques:

Journal:

Article Title: Divalent ion block of inward rectifier current in human capillary endothelial cells and effects on resting membrane potential

doi: 10.1111/j.1469-7793.1998.119bf.x

Figure Lengend Snippet: A, IK(IR) traces during strong hyperpolarizing pulses (−180 to +20 mV) from a holding potential of −60 mV from a HCEC perfused with standard external solution. B, current traces from the same cell in the presence of 5 mm Sr2+. Both the transient and steady-state components of the inward current were blocked. C, current-voltage plot for the peak current (▪) and the current at the end of the pulse (•) in standard bath solution, and the peak current (□) and the steady-state current (○) in 5 mm Sr2+.

Article Snippet: Cell culture Human capillary endothelial cells (HCEC) were obtained from Cell Systems Corporation (WA, USA), and were cultured in growth medium for human capillary endothelial cells (4M1-500, Cell Systems Corporation).

Techniques: